Mobility-based wall adsorption isotherms for comparing capillary electrophoresis with single-molecule observations

TitleMobility-based wall adsorption isotherms for comparing capillary electrophoresis with single-molecule observations
Publication TypeJournal Article
Year of Publication2007
AuthorsFang N, Zhang H, Li JW, Li HW, Yeung ES
Journal TitleAnalytical Chemistry
Volume79
Pages6047-6054
Date PublishedAug
Type of ArticleArticle
ISBN Number0003-2700
Accession NumberISI:000248993300001
KeywordsBINDING CONSTANTS, DNA-MOLECULES, dynamics, ERROR, fluorescence detection, interface, microscopy, monte-carlo-simulation, propagation, RECTANGULAR HYPERBOLAE, ZONE-ELECTROPHORESIS
Abstract

The adsorption properties of R-phycoerythrin (RPE), an autofluorescent protein, on the fused-silica surface were studied in capillary electrophoresis (CE) and in singlemolecule experiments. The band shapes and migration times were measured in CE, and adsorption and desorption events were recorded at the single-molecule level by imaging within the evanescent field layer using total internal reflection fluorescence microscopy. The adsorbed RPE molecules on the surface of the fused-silica prism were counted with confidence based on lmageJ software. The capacity factor and desorption rate were estimated from the counting results. The mobility-based adsorption isotherms were constructed from both computer simulations and experiments to determine the capacity factor.

DOI10.1021/ac071009n
Alternate JournalAnal. Chem.