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Tryptophan and ATTO 590: Mutual Fluorescence Quenching and Exciplex Formation

TitleTryptophan and ATTO 590: Mutual Fluorescence Quenching and Exciplex Formation
Publication TypeJournal Article
Year of Publication2014
AuthorsBhattacharjee, U, Beck, C, Winter, A, Wells, C, Petrich, JW
JournalJournal of Physical Chemistry B
Date Published07
Type of ArticleArticle
ISBN Number1520-6106
Accession NumberWOS:000339540600002
Keywordsaqueous-solution, behavior, density, dynamics, fluorophores, indole, organic-dyes, Spectroscopy

Investigation of fluorescence quenching of probes, such as ATTO dyes, is becoming an increasingly important topic owing to the use of these dyes in super-resolution microscopies and in single-molecule studies. Photoinduced electron transfer is their most important nonradiative pathway. Because of the increasing frequency of the use of ATTO and related dyes to investigate biological systems, studies are presented for inter- and intramolecular quenching of ATTO 590 with tryptophan. In order to examine intramolecular quenching, an ATTO 590 tryptophan conjugate was synthesized. It was determined that tryptophan is efficiently quenching ATTO 590 fluorescence by excited-state charge transfer and two charge transfer complexes are forming. In addition, it was discovered that an exciplex (whose lifetime is 5.6 ns) can be formed between tryptophan and ATTO 590, and it is suggested that the possibility of such exciplex formation should be taken into account when protein fluorescence is monitored in a system tagged with ATTO dyes.

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