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Three-dimensional visualization of membrane phospholipid distributions in Arabidopsis thaliana seeds: A spatial perspective of molecular heterogeneity

TitleThree-dimensional visualization of membrane phospholipid distributions in Arabidopsis thaliana seeds: A spatial perspective of molecular heterogeneity
Publication TypeJournal Article
Year of Publication2017
AuthorsSturtevant, D, Duenas, ME, Lee, YJ, Chapman, KD
JournalBiochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids
Volume1862
Pagination268-281
Date Published02
Type of ArticleArticle
ISBN Number1388-1981
Accession NumberWOS:000392898100013
Keywords3d, arabidopsis, Biophysics, biosynthesis, cell biology, gene, in-situ, ionization mass-spectrometry, lipids, MALDI-MSI, Metabolites, Molecular Biology, ms, mutants, phosphatidylcholine, Phosphatidylinositol, plants, resolution
Abstract

imaging (MALDI-MSI) to map and visualize the three-dimensional spatial distributions of two common membrane phospholipid classes, phosphatidylcholine (PC) and phosphatidylinositol (PI), in single A. thaliana seeds. The 3D images revealed distinct differences in distribution of several molecular species of both phospholipids among different seed tissues. Using data from these 3D reconstructions, the PC and PI mol% lipid profiles were calculated for the embryonic axis, cotyledons, and peripheral endosperm, and these data agreed well with overall quantification of these lipids in bulk seed extracts analyzed by conventional electrospray ionization-mass spectrometry (ESI-MS). In addition, MALDI-MSI was used to profile PC and PI molecular species in seeds of wild type, fad2-1, fad3-2,fad6-1, and fae1-1 acyl lipid mutants. The resulting distributions revealed previously unobserved changes in spatial distribution of several lipid molecular species, and were used to suggest new insights into biochemical heterogeneity of seed lipid metabolism. These studies highlight the value of mass spectrometry imaging to provide unprecedented spatial and chemical resolution of metabolites directly in samples even as small as a single A. thaliana seeds, and allow for expanded imaging of plant metabolites to improve our understanding of plant lipid metabolism from a spatial perspective. (C) 2016 Elsevier B.V. All rights reserved.

DOI10.1016/j.bbalip.2016.11.012
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Metabolites

Short TitleBiochim. Biophys. Acta Mol. Cell Biol. Lipids
Alternate JournalBiochim. Biophys. Acta Mol. Cell Biol. Lipids