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The effect of polarized light on the organization of collagen secreted by fibroblasts

TitleThe effect of polarized light on the organization of collagen secreted by fibroblasts
Publication TypeJournal Article
Year of Publication2018
AuthorsAkilbekova, D, Boddupalli, A, Bratlie, KM
JournalLasers in Medical Science
Volume33
Pagination539-547
Date Published04
Type of ArticleArticle
ISBN Number0268-8921
Accession NumberWOS:000428056300011
Keywords2nd-harmonic generation, Collagen, Engineering, fibroblasts, foreign-body reaction, irradiation, level laser therapy, mechanism, radiation, Regeneration, Second harmonic generation, skin, Surgery, tissue, vegf
Abstract

Recent studies have demonstrated the beneficial effect of low-power lasers and polarized light on wound healing, inflammation, and the treatment of rheumatologic and neurologic disorders. The overall effect of laser irradiation treatment is still controversial due to the lack of studies on the biochemical mechanisms and the optimal parameters for the incident light that should be chosen for particular applications. Here, we study how NIH/3T3 fibroblasts respond to irradiation with linearly polarized light at different polarization angles. In particular, we examined vascular endothelial growth factor (VEGF) secretion, differentiation to myofibroblasts, and collagen organization in response to 800 nm polarized light at 0A degrees, 45A degrees, 90A degrees, and 135A degrees with a power density of 40 mW/cm(2) for 6 min every day for 6 days. Additional experiments were conducted in which the polarization angle of the incident was changed every day to induce an isotropic distribution of collagen. The data presented here shows that polarized light can upregulate VEGF production, myofibroblast differentiation, and induce different collagen organization in response to different polarization angles of the incident beam. These results are encouraging and demonstrate possible methods for controlling cell response through the polarization angle of the laser light, which has potential for the treatment of wounds.

DOI10.1007/s10103-017-2398-0
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