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Stochastic Optical Reconstruction Microscopy Imaging of Microtubule Arrays in Intact Arabidopsis thaliana Seedling Roots

TitleStochastic Optical Reconstruction Microscopy Imaging of Microtubule Arrays in Intact Arabidopsis thaliana Seedling Roots
Publication TypeJournal Article
Year of Publication2015
AuthorsDong, B, Yang, XC, Zhu, SB, Bassham, DC, Fang, N
JournalScientific Reports
Volume5
Pagination15694
Date Published10
Type of ArticleArticle
ISBN Number2045-2322
Accession NumberWOS:000363447900002
Keywordscortical, cytoskeletal control, fluorescent-probes, growth, microtubules, organization, plant-cells, plasma-membrane, structured-illumination microscopy, superresolution, thick biological samples
Abstract

Super-resolution fluorescence microscopy has generated tremendous success in revealing detailed subcellular structures in animal cells. However, its application to plant cell biology remains extremely limited due to numerous technical challenges, including the generally high fluorescence background of plant cells and the presence of the cell wall. In the current study, stochastic optical reconstruction microscopy (STORM) imaging of intact Arabidopsis thaliana seedling roots with a spatial resolution of 20-40 nm was demonstrated. Using the super-resolution images, the spatial organization of cortical microtubules in different parts of a whole Arabidopsis root tip was analyzed quantitatively, and the results show the dramatic differences in the density and spatial organization of cortical microtubules in cells of different differentiation stages or types. The method developed can be applied to plant cell biological processes, including imaging of additional elements of the cytoskeleton, organelle substructure, and membrane domains.

DOI10.1038/srep15694
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