|Title||Simultaneous Single-Particle Superlocalization and Rotational Tracking|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Gu, Y, Wang, GF, Fang, N|
|Type of Article||Article|
|Keywords||cargo transport, fluorescence, gold nanorods, imaging, interference contrast microscopy, kinesin, live cells, microscopy, molecular motors, myosin-v, optical reconstruction microscopy, photoactivated localization, polarization, rotational, secretory granules, superlocalization, tracking|
Super localization of single molecules and nanoparticles has become an essential procedure to bring new insights into nanoscale structures and dynamics of biological systems. In the present study, superlocalization is combined with the newly introduced differential interference contrast (DIC) microscopy-based single-particle orientation and rotational tracking. The new technique overcomes the difficulty in localization of the antisymmetric DIC point spread function by using a dual-modality microscope configuration for simultaneous rotational tracking and localization of single gold nanorods with nanometer-scale precision. The new imaging setup has been applied to study the steric hindrance induced by relatively large cargos in the microtubule gliding assay and to track nanocargos in the crowded cellular environment. This technique has great potential in the study of biological processes where both localization and rotational information are required.
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