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pH sensitive methacrylated chitosan hydrogels with tunable physical and chemical properties

TitlepH sensitive methacrylated chitosan hydrogels with tunable physical and chemical properties
Publication TypeJournal Article
Year of Publication2018
AuthorsZhu, LD, Bratlie, KM
JournalBiochemical Engineering Journal
Volume132
Pagination38-46
Date Published04
Type of ArticleArticle
ISBN Number1369-703X
Accession NumberWOS:000433266500005
Keywordsadipose-tissue, alginate hydrogels, Applied Microbiology, Chitosan, Click chemistry, cross-linker type, Engineering, fibroblasts, hyaluronic-acid, mechanical-properties, pH responsive polymers, photocrosslinkable chitosan, polymer network, swelling properties, tissue engineering applications, youngs modulus
Abstract

n be developed to respond to these environmental cues using pH sensitive hydrogels. To obtain tunable physical and chemical properties, chitosan was first methacrylated and then crosslinked through three polymerization methods step growth by a thiol-ene photoclick reaction, chain growth by UV polymerization, and mixed mode in which both step growth and chain growth mechanisms were used. pH sensitive methacrylated chitosan (MAC) hydrogels were synthesized and confirmed through 1H NMR. The resulting hydrogels exhibited adjustable mechanical properties, swelling ratios, and pH sensitivities without affecting degradation behavior or in vitro cell responses. Cytocompatibility studies were performed using NIH/3T3 fibroblasts. Cell proliferation or adhesion was suppressed when seeded on the hydrogel surfaces compared to tissue culture plastic (TCP), yet no measureable cell death was observed. The responsivity of these gels to changing pH environments may prove useful as stage-responsive wound dressings. (C) 2018 Elsevier B.V. All rights reserved.

DOI10.1016/j.bej.2017.12.012
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