You are here

Matrix Recrystallization for MALDI-MS Imaging of Maize Lipids at High-Spatial Resolution

TitleMatrix Recrystallization for MALDI-MS Imaging of Maize Lipids at High-Spatial Resolution
Publication TypeJournal Article
Year of Publication2016
AuthorsDuenas, ME, Carlucci, L, Lee, YJ
JournalJournal of the American Society for Mass Spectrometry
Volume27
Pagination1575-1578
Date Published09
Type of ArticleArticle
ISBN Number1044-0305
Accession NumberWOS:000381278500016
Keywordschemistry, efficiency, High spatial resolution, latent fingermarks, lipids, Maize, MALDI, mass spectrometry imaging, mass-spectrometry, matrix, Metabolites, Molecular Biology, recrystallization, Spectroscopy, sublimation, tissue, tof ms
Abstract

Matrix recrystallization is optimized and applied to improve lipid ion signals in maize embryos and leaves. A systematic study was performed varying solvent and incubation time. During this study, unexpected side reactions were found when methanol was used as a recrystallization solvent, resulting in the formation of a methyl ester of phosphatidic acid. Using an optimum recrystallization condition with isopropanol, there is no apparent delocalization demonstrated with a transmission electron microscopy (TEM) pattern and maize leaf images obtained at 10 mu m spatial resolution.

DOI10.1007/s13361-016-1422-0
Custom 1

Plant Metabolites

Custom 2

SULI

Short TitleJ. Am. Soc. Mass Spectrom.
Alternate JournalJ. Am. Soc. Mass Spectrom.