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Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ

TitleMatrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ
Publication TypeJournal Article
Year of Publication2016
AuthorsSturtevant, D, Lee, YJ, Chapman, KD
JournalCurrent Opinion in Biotechnology
Volume37
Pagination53-60
Date Published02
Type of ArticleReview
ISBN Number0958-1669
Accession NumberWOS:000370458800008
Keywordsarabidopsis-thaliana, ionization, localization, nitrogen-fixation, proteins, resolution, seeds, surface metabolites, tissue, tof ms
Abstract

ls. Strategies have been applied in many areas of metabolism including isotope flux analyses, plant interactions, and transcriptional regulation of metabolite accumulation. Technological advances have pushed achievable spatial resolution to subcellular levels and increased instrument sensitivity by several orders of magnitude. It is anticipated that MALDI-MSI and other MSI approaches will bring a new level of understanding to metabolomics as scientists will be encouraged to consider spatial heterogeneity of metabolites in descriptions of metabolic pathway regulation.

DOI10.1016/j.copbio.2015.10.004
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Plant Metabolites