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Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters

TitleLive-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters
Publication TypeJournal Article
Year of Publication2014
AuthorsShin, I, Ray, J, Gupta, V, Ilgu, M, Beasley, J, Bendickson, L, Mehanovic, S, Kraus, G, Nilsen-Hamilton, M
JournalNucleic Acids Research
Volume42
Paginatione90
Date Published04
Type of ArticleArticle
ISBN Number0305-1048
Accession NumberWOS:000338769400002
Keywordsbacteria, green fluorescent protein, living cells, magnesium, messenger-rnas, transcription, translation, visualization, yeast
Abstract

eign proteins are produced in the cells that could be toxic or otherwise influence the cellular response as they accumulate, the IMAGEtags are short lived and oxygen is not required to generate their signals. The IMAGEtag RNA reporter system provides a means of tracking changes in transcriptional activity in live cells and in real time.

DOI10.1093/nar/gku297
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Imaging Gene Expression

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