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Light-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon scutellarioides (Coleus Henna) Visualized by Matrix-Free Atmospheric Pressure Electrospray Laser Desorption Ionization Mass Spectrometry Imaging

TitleLight-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon scutellarioides (Coleus Henna) Visualized by Matrix-Free Atmospheric Pressure Electrospray Laser Desorption Ionization Mass Spectrometry Imaging
Publication TypeJournal Article
Year of Publication2018
AuthorsMcVey, PA, Alexander, LE, Fu, XY, Xie, B, Galayda, KJ, Nikolau, BJ, Houk, RS
JournalFrontiers in Plant Science
Volume9
Pagination1348
Date Published09
Type of ArticleArticle
ISBN Number1664-462X
Accession NumberWOS:000444984400001
Keywordsanthocyanin, anthocyanins, arabidopsis-thaliana, Coleus (Solenostemon scuttellarioides), desorption ionization, electrospray laser, flavonoid, in-vivo, leaves, mass spectrometry imaging, matrix-free imaging, photoprotection, photosynthesis, plant metabolomics, Plant Sciences, shading, sink-regulation, source-to-sink, sugar, temperature
Abstract

The visualization of foliage color in plants provides immediate insight into some of the compounds that exist in the leaf. However, many non-colored compounds are also present; their cellular distributions are not readily identifiable optically. In this study we evaluate the applicability of mass spectrometry imaging (MSI) via electrospray laser desorption ionization (ELDI) to reveal the spatial distribution of metabolites. ELDI-MSI is a matrix free, atmospheric pressure ionization method that utilizes a UV laser coupled with supplemental ionization by electrospray. We specifically applied ELDI-MSI to determine the spatial distribution of metabolites in Coleus Henna half leaves that were grown with half-sections either fully illuminated or shaded. We monitored dynamic changes in the spatial distribution of metabolites in response to the change of illumination every 7 days for a 28 day period. A novel source-sink relationship was observed between the 2 halves of the experimental leaf. Furthermore, Coleus Henna leaves present visually recognizable sectors associated with the differential accumulation of flavonoids. Thus, we correlated the effect of differential illumination and presence or absence of flavonoids with metabolic changes revealed by the accumulation of carbohydrates, amino acids, and organic acids. The results show the potential of ELDI-MSI to provide spatial information for a variety of plant metabolites with little sample preparation.

DOI10.3389/fpls.2018.01348
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Short TitleFront. Plant Sci.
Alternate JournalFront. Plant Sci.