|Title||High-Throughput Analysis of Algal Crude Oils Using High Resolution Mass Spectrometry|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Lee, YJ, Leverence, RC, Smith, EA, Valenstein, JS, Kandel, K, Trewyn, BG|
|Type of Article||Article|
|Keywords||Analytical chemistry, Analytical techniques, biodiesel, delivery-system, fatty-acids, feedstock, ionization, lipids, Mass spectrometry (MS), microalgae, nanoparticles, responsive controlled-release, sequestration|
protocol to monitor various lipid classes in algal crude oils. Atmospheric pressure chemical ionization was determined to be most effective for this purpose to cover a wide range of lipid classes. With an autosampler-LC pump set-up, we could analyze algal crude samples every one and half minutes, monitoring several lipid species such as TAG, DAG, squalene, sterols, and chlorophyll a. High-mass resolution and high-mass accuracy of the orbitrap mass analyzer provides confidence in the identification of these lipid compounds. MS/MS and MS3 analysis could be performed in parallel for further structural information, as demonstrated for TAG and DAG. This high-throughput method was successfully demonstrated for semi-quantitative analysis of algal oils after treatment with various nanoparticles.
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