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High spatial resolution mass spectrometry imaging reveals the genetically programmed, developmental modification of the distribution of thylakoid membrane lipids among individual cells of maize leaf

TitleHigh spatial resolution mass spectrometry imaging reveals the genetically programmed, developmental modification of the distribution of thylakoid membrane lipids among individual cells of maize leaf
Publication TypeJournal Article
Year of Publication2017
AuthorsDuenas, ME, Klein, AT, Alexander, LE, Yandeau-Nelson, MD, Nikolau, BJ, Lee, YJ
JournalPlant Journal
Volume89
Pagination825-838
Date Published02
Type of ArticleArticle
ISBN Number0960-7412
Accession NumberWOS:000395802500013
KeywordsB73, bundle sheath, bundle-sheath cells, c-4 plants, c4 plants, chilling sensitivity, comparative proteomics, evolving photosystem-ii, fatty-acid-composition, in-situ, ion-trap, Kranz anatomy, mass spectrometry imaging, mesophyll, Mo17, phosphatidylglycerol, Plant Sciences, single cell, Zea mays L.
Abstract

3). SQDG species are uniformly distributed in both photosynthetic cell types, regardless of leaf development or genotype; however, PG shows photosynthetic cell-specific differential localization depending on the genotype and the fatty acyl chain constituent. Overall, 16:1-containing PGs primarily contribute to the thylakoid membranes of M cells, whereas BS chloroplasts are mostly composed of 16:0-containing PGs. Furthermore, PG32:0 shows genotype-specific differences in cellular distribution, with preferential localization in BS cells for B73, but more uniform distribution between BS and M cells in Mo17. Maternal inheritance is exhibited within the hybrids, such that the localization of PG 32:0 in B73xMo17 is similar to the distribution in the B73 parental inbred, whereas that of Mo17xB73 resembles the Mo17 parent. This study demonstrates the power of MALDI-MSI to reveal unprecedented insights on metabolic outcomes in multicellular organisms at single-cell resolution.

DOI10.1111/tpj.13422
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Plant Metabolites

Short TitlePlant J.
Alternate JournalPlant J.