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Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System

TitleFive Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System
Publication TypeJournal Article
Year of Publication2017
AuthorsFeenstra, AD, Duenas, ME, Lee, YJ
JournalJournal of the American Society for Mass Spectrometry
Volume28
Pagination434-442
Date Published03
Type of ArticleArticle
ISBN Number1044-0305
Accession NumberWOS:000398111400005
Keywordsbeam, chemistry, Laser spot size, laser-desorption-ionization, Maize, MALDI, mass spectrometry imaging, Metabolites, Molecular Biology, ms, optics, plant metabolomics, profile, resolution, Root, Spectroscopy, spot size, subcellular spatial-resolution, tissue-sections, transmission geometry
Abstract

mplished through a combination of spatial filtering, beam expansion, and reduction of the final focal length. Most importantly, the new laser optics system allows for simple modification of the spot size solely through the interchanging of the beam expander component. Using 10x, 5x, and no beam expander, we could routinely change between similar to 4, similar to 7, and similar to 45 mu m laser spot size, in less than 5 min. We applied this multi-resolution MALDI-MSI system to a single maize root tissue section with three different spatial resolutions of 5, 10, and 50 mu m and compared the differences in imaging quality and signal sensitivity. We also demonstrated the difference in depth of focus between the optical systems with 10x and 5x beam expanders.

DOI10.1007/s13361-016-1577-8
Custom 1

Plant Metabolites

Short TitleJ. Am. Soc. Mass Spectrom.
Alternate JournalJ. Am. Soc. Mass Spectrom.