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CRISPR-Mediated Genome Editing and Gene Repression in Scheffersomyces stipitis

TitleCRISPR-Mediated Genome Editing and Gene Repression in Scheffersomyces stipitis
Publication TypeJournal Article
Year of Publication2018
AuthorsCao, MF, Gao, MR, Ploessl, D, Song, CJ, Shao, ZY
JournalBiotechnology Journal
Volume13
Pagination1700598
Date Published09
Type of ArticleArticle
ISBN Number1860-6768
Accession NumberWOS:000443381400012
KeywordsApplied Microbiology, aromatic-compounds, CRISPR, expression, gene transcription regulation, homologous, homologous recombination, integration, ku70ku80 deficiency, mammalian-cells, Molecular Biology, non-homologous end joining, overexpression, recombination, saccharomyces-cerevisiae, sequence, transcription, yeast pichia-stipitis
Abstract

obial host, the gene knockdown tool is also established, and repression efficiency is improved by incorporating a transcriptional repressor Mxi1 into the CRISPR-dCas9 platform. All these results are obtained with the improved transformation efficiency, which is 191-fold higher than that obtained with the traditional parameters used in yeast transformation. This work paves the way for advancing a new microbial chassis and provides a guideline for developing efficient CRISPR tools in other nonconventional yeasts.

DOI10.1002/biot.201700598
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Short TitleBiotechnol. J.
Alternate JournalBiotechnol. J.