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Cellular and Subcellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution MALDI Mass Spectrometry Imaging

TitleCellular and Subcellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution MALDI Mass Spectrometry Imaging
Publication TypeJournal Article
Year of Publication2018
AuthorsDuenas, ME, Feenstra, AD, Korte, AR, Hinners, P, Lee, YJ
Secondary AuthorsLagrimini, LM
JournalMaize: Methods and Protocols
Volume1676
Pagination217-231
Date Published05
ISBN Number978-1-4939-7315-6; 978-1-4939-7314-9
Accession NumberWOS:000432298100014
Keywordscells, High-spatial resolution, leaf, lipids, MALDI, mass spectrometry imaging, matrix, Metabolites, ms, tissue-sections
Abstract

Recent technological advances have pushed the achievable spatial resolution for mass spectrometry imaging (MSI) to cellular and subcellular levels. Direct visualization of maize tissues by this tool has provided key insights into the localization of metabolites and lipids. This chapter outlines methodology for sample preparation, data acquisition, and data analysis of maize tissue sections using high-spatial resolution matrix-assisted laser desorption ionization (MALDI)-MSI, as well as the incorporation of a multi-resolution optical system, which allows for simple inter-conversion between different resolution setups (5, 10, and 50 mu m imaging).

DOI10.1007/978-1-4939-7315-6_13
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Short TitleMethods Mol. Biol.