ref1.4.c - Reflectivity Curve Function

What is ref1.4.c?

ref1.4.c is a C-Plot function that calculates the reflectivity curve for a given set of parameters supplied by parameters.dat in the current directory.

Function Specifics:

Experiment Data Sheet, Liquid Surface Reflectometer

Spread the Monolayer

  • Gloves are required and be sure the hood in the lab room is on.
  • Fill the trough in the spectrometer with water obtained from the Milli-Q machine.
  • Pour the water onto the glass area at a steady rate (to avoid splashing), until the water level is above the glass.
  • Remove any excess water. The optimum water level is one that is just above the glass surface, so any motion waves on the surface of the water are dampened when they reach the area over the glass.

Prepare a Sample Solution

These instructions are for the preparation of arachidic acid. Adjustments may be made for the preparation of other chemicals.


Wear gloves, turn the hood on.
Use a clean vial and a micro-spatula cleaned with methanol.

Cleaning Vials

  • Put on gloves and aquire two 600 mL containers (one labeled soap, one labeled water) and the vials/flasks to be cleaned.
  • Use pure water from the Milli-Q purification machine.
  • Rinse containers and put the vials in the soap container.
  • Pour a small (2% of total volume) amount of Hellmanex cleaner into the soap container and fill.
  • Use necessary precautions associated with Hellmanex.
  • Put the soap container in the sonicator and fill the bath.
  • Turn it on and clean the vials fo

Milli-Q Machine

  • Press "operate/standby" button to turn the machine on.
  • Display should show 18.2 MOhm cm (this is resistivity for pure water).
  • Remove cap from water outlet.
  • Turn knob from "recirculation" to "production."
  • Let water run for 1 - 2 minutes to ensure pureness.
  • Collect water.
  • Turn knob back to "recirculation."
  • Press "operate/standby" button to turn it off.

Startup Procedure for the X-Ray Generator

  • Open water valves for the heat exchanger. These are the two valves on the facility water pipes.
  • Turn on the heat exchanger. Make sure the level of flow is at 8 gallons per minute, the pressure is 50 psi, and the temperature is set to 65 degrees F.
  • Turn on breaker on back panel of the x-ray generator.
  • Turn on the vacum pump (this is the "start" button on the front panel of the generator). The pump light should flash. Use the cursor on the front panel to "Check IG".

DON'T turn on x-rays until IG reads < 150mV


Sphingolipids are an essential part to many cell membranes. They are all derived from a base lipid named sphingosine, which is composed of an 18 carbon chain molecule with hydroxyl and amine groups:


Sphingolipids are present in skin, milk, egg, brain, and are believed to be present in every living cell. Not only are they functional parts of the membrane, but they also act as messengers for cell apoptosis, proliferation, and differentiation. We have been studying a variety of sphingolipids. Below we show some results concerning ceramides.

Electrochemistry at the Air-Water Interface

2D Crystallization of a Pigment Array Contiguous to a Langmuir Monolayer for Light Harvesting Antenna

Title of Experiment:  
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