Three-Dimensional Orientation Determination of Stationary Anisotropic Nanoparticles with Sub-Degree Precision under Total Internal Reflection Scattering Microscopy

TitleThree-Dimensional Orientation Determination of Stationary Anisotropic Nanoparticles with Sub-Degree Precision under Total Internal Reflection Scattering Microscopy
Publication TypeJournal Article
Year of Publication2013
AuthorsMarchuk K, Fang N
Journal TitleNano Letters
Volume13
Pages5414-5419
Date Published11
Type of ArticleArticle
ISBN Number1530-6984
Accession NumberWOS:000327111700066
Keywordsgold nanorods, interference contrast microscopy, localized surface plasmon, microtubule cargo, orientation determination, RESONANCE, rotational-dynamics, sensors, single-particle, Total internal reflection scattering (TIRS), TRACKING
Abstract

Single-particle and single-molecule orientation determination plays a vital role in deciphering nanoscale motion in complex environments. Previous attempts to determine the absolute three-dimensional orientation of anisotropic particles rely on subjective pattern matching and are inherently plagued by high degrees of uncertainty. Herein, we describe a method utilizing total internal reflection scattering microscopy to determine the 3D orientation of gold nanorods with subdegree uncertainty. The method is then applied to the biologically relevant system of microtubule cargo loading. Finally, we demonstrate the method holds potential for identifying single particles versus proximate neighbors within the diffraction limited area.

URL<Go to ISI>://WOS:000327111700066
DOI10.1021/nl4029818