Analysis of Resistant Starches in Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy

TitleAnalysis of Resistant Starches in Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy
Publication TypeJournal Article
Year of Publication2013
AuthorsAnderson TJ, Ai YF, Jones RW, Houk RS, Jane JL, Zhao YS, Birt DF, McClelland JF
Journal TitleJournal of Agricultural and Food Chemistry
Volume61
Pages1818-1822
Date Published02
Type of ArticleArticle
ISBN Number0021-8561
Accession NumberWOS:000315619000026
Keywordsamylose, analysis, components, dietary fiber, fecal analysis, Fourier transform infrared, GLUCOSE, metabolism, Partial least squares, photoacoustic spectroscopy, Principal component, resistant starch
Abstract

Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) qualitatively and quantitatively measured resistant starch (RS) in rat cecal contents. Fisher 344 rats were fed diets of 55% (w/w, dry basis) starch for 8 weeks. Cecal contents were collected from sacrificed rats. A corn starch control was compared against three RS diets. The RS diets were high-amylose corn starch (HA7), HA7 chemically modified with octenyl succinic anhydride, and stearic-acid-complexed HA7 starch. To calibrate the FTIR-PAS analysis, samples from each diet were analyzed using an enzymatic assay. A partial least-squares cross-validation plot generated from the enzymatic assay and FTIR-PAS spectral results for starch fit the ideal curve with a R-2 of 0.997. A principal component analysis plot of components 1 and 2 showed that spectra from diets clustered significantly from each other. This study clearly showed that FTIR-PAS can accurately quantify starch content and identify the form of starch in complex matrices.

URL<Go to ISI>://WOS:000315619000026
DOI10.1021/jf3042616