Three-Dimensional Super-Localization and Tracking of Single Gold Nanoparticles in Cells

TitleThree-Dimensional Super-Localization and Tracking of Single Gold Nanoparticles in Cells
Publication TypeJournal Article
Year of Publication2012
AuthorsGu Y, Di XW, Sun W, Wang GF, Fang N
Journal TitleAnalytical Chemistry
Date Published05
Type of ArticleArticle
ISBN Number0003-2700
Accession NumberWOS:000303349200036
Keywordsdrug-delivery, dynamics, fluorescent-probes, IN-VIVO, interference-contrast microscopy, live cells, particle tracking, quantum dots, receptor-mediated endocytosis, surface-plasmon resonance

We introduce a precise three-dimensional (3D) localization method of spherical gold nanoparticle probes using model-based correlation coefficient mapping. To accomplish this, a stack of sample images at different z-positions are acquired, and a 3D intensity profile of the probe serving as the model is used to map out the positions of nanoparticles in the sample. By using this model-based correlation imaging method, precise localization can be achieved in imaging techniques with complicated point spread functions (PSF) such as differential interference contrast (DIC) microscopy. We demonstrated the localization precision of 4-7 nm laterally and 16 nm axially for 40-nm gold nanospheres at an imaging rate of 10 frames per second. The 3D superlocalization method was applied to tracking gold nanospheres during live endocytosis events.

URL<Go to ISI>://WOS:000303349200036
Alternate JournalAnal. Chem.