Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton

TitleSpatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton
Publication TypeJournal Article
Year of Publication2012
AuthorsHorn PJ, Korte AR, Neogi PB, Love E, Fuchs J, Strupat K, Borisjuk L, Shulaev V, Lee YJ, Chapman KD
Journal TitlePlant Cell
Volume24
Pages622-636
Date Published02
Type of ArticleArticle
ISBN Number1040-4651
Accession NumberWOS:000302131000019
KeywordsARABIDOPSIS-THALIANA, biological, cyclopropenoid fatty-acids, ion-cyclotron resonance, ionization mass-spectrometry, maldi-tof ms, PERFORMANCE LIQUID-CHROMATOGRAPHY, phosphatidic-acid, plant metabolites, SAMPLES, vegetable-oils
Abstract

Advances in mass spectrometry (MS) have made comprehensive lipidomics analysis of complex tissues relatively commonplace. These compositional analyses, although able to resolve hundreds of molecular species of lipids in single extracts, lose the original cellular context from which these lipids are derived. Recently, high-resolution MS of individual lipid droplets from seed tissues indicated organelle-to-organelle variation in lipid composition, suggesting that heterogeneity of lipid distributions at the cellular level may be prevalent. Here, we employed matrix-assisted laser desorption/ionization-MS imaging (MALDI-MSI) approaches to visualize lipid species directly in seed tissues of upland cotton (Gossypium hirsutum). MS imaging of cryosections of mature cotton embryos revealed a distinct, heterogeneous distribution of molecular species of triacylglycerols and phosphatidylcholines, the major storage and membrane lipid classes in cotton embryos. Other lipids were imaged, including phosphatidylethanolamines, phosphatidic acids, sterols, and gossypol, indicating the broad range of metabolites and applications for this chemical visualization approach. We conclude that comprehensive lipidomics images generated by MALDI-MSI report accurate, relative amounts of lipid species in plant tissues and reveal previously unseen differences in spatial distributions providing for a new level of understanding in cellular biochemistry.

URL<Go to ISI>://WOS:000302131000019
DOI10.1105/tpc.111.094581
Alternate JournalPlant Cell