High-Precision Tracking with Non-blinking Quantum Dots Resolves Nanoscale Vertical Displacement

TitleHigh-Precision Tracking with Non-blinking Quantum Dots Resolves Nanoscale Vertical Displacement
Publication TypeJournal Article
Year of Publication2012
AuthorsMarchuk K, Guo YJ, Sun W, Vela J, Fang N
Journal TitleJournal of the American Chemical Society
Date Published04
Type of ArticleArticle
ISBN Number0002-7863
Accession NumberWOS:000302524800014
Keywordsangle, cargo, motility, reflection fluorescence microscopy, resolution, SAMPLES, SEMICONDUCTOR NANOCRYSTALS, suppression, TRANSPORT

Novel non-blinking quantum dots (NBQDs) were utilized in three-dimensional super-localization, high-precision tracking applications under an automated scanning-angle total internal reflection fluorescence microscope (SA-TIRFM). NBQDs were randomly attached to stationary microtubules along the radial axis under gliding assay conditions. By automatically scanning through a wide range of incident angles with different evanescent-field layer thicknesses, the fluorescence intensity decay curves were obtained. Fit with theoretical decay functions, the absolute vertical positions were determined with sub-10-nm localization precision. The emission intensity profile of the NBQDs attached to kinesin-propelled microtubules was used to resolve the self-rotation of gliding microtubules within a small vertical distance of similar to 50 nm. We demonstrate the applicability of NBQDs in high-precision fluorescence imaging experiments.

URL<Go to ISI>://WOS:000302524800014
Alternate JournalJ. Am. Chem. Soc.