Dual-Wavelength Detection of Rotational Diffusion of Single Anisotropic Nanocarriers on Live Cell Membranes

TitleDual-Wavelength Detection of Rotational Diffusion of Single Anisotropic Nanocarriers on Live Cell Membranes
Publication TypeJournal Article
Year of Publication2012
AuthorsHa JW, Sun W, Stender AS, Fang N
Journal TitleJournal of Physical Chemistry C
Date Published02
Type of ArticleArticle
ISBN Number1932-7447
Accession NumberWOS:000300462400015
KeywordsABSORPTION, aspect ratio, dark-field microscopy, drug-delivery system, dynamics, gold nanorods, METAL NANOPARTICLES, RELEASE, spectroscopy, surface-plasmon resonance

Single-particle rotational tracking is of great importance to monitor orientation changes of biomolecules and to understand their functions and mechanisms in biological systems. Differential interference contrast (DIC) microscopy has been found to be an excellent tool to measure polarization anisotropy for tracking rotational dynamics of gold nanorod (AuNR) probes. DIC polarization anisotropy can be conveniently obtained from the bright and dark intensities of a single DIC image of an AuNR. Here, DIC microscopy-based dual-wavelength detection of rotational motions of AuNRs at both transverse and longitudinal surface plasmon resonance (SPR) wavelengths is demonstrated. The transverse SPR mode was successfully used to track fast rotational dynamics of individual AuNRs on live cell membranes. This is important since the transverse SPR mode is mostly insensitive to the medium refractive index, AuNR aspect ratio, and adsorption of biomolecules. DIC polarization anisotropy was simultaneously obtained from the two SPR wavelengths during the dynamic process. wavelengths showed good agreement and provided accurate and reliable measurement of AuNR orientation. Both

URL<Go to ISI>://WOS:000300462400015
Alternate JournalJ. Phys. Chem. C