Ordered Mesoporous Polymer-Silica Hybrid Nanoparticles as Vehicles for the Intracellular Controlled Release of Macromolecules

TitleOrdered Mesoporous Polymer-Silica Hybrid Nanoparticles as Vehicles for the Intracellular Controlled Release of Macromolecules
Publication TypeJournal Article
Year of Publication2011
AuthorsKim TW, Slowing II, Chung PW, Lin VSY
Journal TitleACS Nano
Volume5
Pages360-366
Date Published01
Type of ArticleArticle
ISBN Number1936-0851
Accession NumberISI:000286487300046
Keywordsadsorption, cellular uptake, controlled release, delivery, drug, drug-delivery, endocytosis, mesoporous polymer-silica composite, mesostructured nanoparticles, MORPHOLOGY, particles, route, sba-15 silica
Abstract

A two-dimensional hexagonal ordered mesoporous polymer-silica hybrid nanoparticle (PSN) material was synthesized by polymerization of acrylate monomers on the surface of SBA-15 mesoporous silica nanoparticles. The structure of the PSN material was analyzed Using a series of different techniques, Including transmission electron microscopy, powder X-ray. diffraction, and N-2 sorption analysis. These structurally ordered, mesoporous polymer-silica hybrid nanoparticles were used for the controlled release Of membrane impermeable macromolecules inside eukaryotic cells. The cellular uptake efficiency and biocompatibility of PSN with human cervical cancer cells (HeLa) were investigated : Our results show that the inhibitory, concentration (IC50) of PSN is very high (>100 mu g/mL per million cells), while the median effective concentration for the uptake (EC50) of PSN is low (EC50 = 44 mu g/mL), indicating that PSNs are fairly biocompatible and easily up taken in vitro. A membrane impermeable macromolecule, 40 kDa FITC-Dextran, was loaded into the mesopores of PSNs at low pH: We demonstrated that the PSN material could indeed serve as a transmembrane carrier for the controlled release of FITC-Dextran at the pH level inside live HeLa cells. We believe that further developments of this PSN material will lead to a new generation of nanodevices for intracellular controlled delivery applications.

URL<Go to ISI>://000286487300046
DOI10.1002/adfm.201001307
Alternate JournalACS Nano