High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver

TitleHigh-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver
Publication TypeJournal Article
Year of Publication2010
AuthorsJun JH, Song ZH, Liu ZJ, Nikolau BJ, Yeung ES, Lee YJ
Journal TitleAnalytical Chemistry
Volume82
Pages3255-3265
Date Published04/15
ISBN Number0003-2700
Accession NumberISI:000276557600023
Keywordsbrain, distributions, MATRIX, molecules, MS, peptides, plant metabolites, POLYMERS, protein expression, TISSUE-SECTIONS
Abstract

High-spatial resolution and high-mass resolution techniques are developed and adopted for the mass spectrometric imaging of epicuticular lipids on the surface of Arabidopsis thaliana. Single cell level spatial resolution of 12 pm was achieved by reducing the laser beam size by using an optical fiber with 25 pm core diameter in a vacuum matrix-assisted laser desorption ionization-linear ion trap (vMALDI-LTQ) mass spectrometer and improved matrix application using an oscillating capillary nebulizer. Fine chemical images of a whole flower were visualized in this high spatial resolution showing substructure of an anther and single pollen grains at the stigma and anthers. The LTQ-Orbitrap with a MALDI ion source was adopted to achieve MS imaging in high mass resolution. Specifically, isobaric silver ion adducts of C29 alkane (m/z 515.3741) and C28 aldehyde (m/z 515.3377), indistinguishable in low-resolution LTQ, can now be clearly distinguished and their chemical images could be separately constructed. In the application to roots, the high spatial resolution allowed molecular MS imaging of secondary roots and the high mass resolution allowed direct identification of lipid metabolites on root surfaces.

URL<Go to ISI>://000276557600023