Mass Spectrometric Imaging of Plant Metabolites

Personnel

Project Leader(s):
Basil Nikolau

Principal Investigators:
Robert Houk, Young-Jin Lee, Basil Nikolau

Overview

We are developing mass spectrometric imaging techniques to map metabolite distributions within plant tissues, and eventually among individual plant cells. Such details will ultimately lead to a predictive understanding of the mechanisms that multicellular organisms use to regulate metabolic processes. By studying the diversity of the cuticular waxes, we hope to gain detailed insight into their biosynthesis as a function of genetics, tissue type, development, and environment. A laser beam will be used to interrogate sequentially micrometer areas of a plant by vaporizing the surface contents of the tissue into a mass spectrometer. Rastering of the laser beam over the tissue will produce a laterally resolved image of the various substances within different structures of the plant. Repeated vaporization at the same focused point of a plant structure will produce a depth profile of the components. We plan to generate ions directly from the plant tissue by designing novel additives as pseudo-matrixes.

 

This research is supported by the U.S. Department of Energy, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences through the Ames Laboratory.  The Ames Laboratory is operated for the U.S. Department of Energy by Iowa State University under Contract No. DE-AC02-07CH11358.

Publications

2013
Korte A R; Lee Y J . 2013. Multiplex Mass Spectrometric Imaging with Polarity Switching for Concurrent Acquisition of Positive and Negative Ion Images. Journal of the American Society for Mass Spectrometry. 24:949-955. abstract
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Yagnik G B; Korte A R; Lee Y J . 2013. Multiplex mass spectrometry imaging for latent fingerprints. Journal of Mass Spectrometry. 48:100-104. abstract
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2012
Smith E A; Park S; Klein A T; Lee Y J . 2012. Bio-oil Analysis Using Negative Electrospray Ionization: Comparative Study of High-Resolution Mass Spectrometers and Phenolic versus Sugaric Components. Energy & Fuels. 26:3796-3802. abstract
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Lee Y J; Perdian D C; Song Z H; Yeung E S; Nikolau B J . 2012. Use of mass spectrometry for imaging metabolites in plants. Plant Journal. 70:81-95. abstract
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Korte A R; Song Z H; Nikolau B J; Lee Y J . 2012. Mass spectrometric imaging as a high-spatial resolution tool for functional genomics: Tissue-specific gene expression of TT7 inferred from heterogeneous distribution of metabolites in Arabidopsis flowers. Analytical Methods. 4:474-481. abstract
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Horn P J; Korte A R; Neogi P B; Love E; Fuchs J; Strupat K; Borisjuk L; Shulaev V; Lee Y J; Chapman K D . 2012. Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton. Plant Cell. 24:622-636. abstract
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2010
Houk R S . 2010. Thirty years ago this month .... Journal of Analytical Atomic Spectrometry. 25:1801-1802. abstract
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Perdian D C; Lee Y J . 2010. Imaging MS Methodology for More Chemical Information in Less Data Acquisition Time Utilizing a Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer. Analytical Chemistry. 82:9393-9400. abstract
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Jun J H; Song Z H; Liu Z J; Nikolau B J; Yeung E S; Lee Y J . 2010. High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver. Analytical Chemistry. 82:3255-3265. abstract
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Perdian D C; Cha S; Oh J; Sakaguchi D S; Yeung E S; Lee Y J . 2010. In situ probing of cholesterol in astrocytes at the single-cell level using laser desorption ionization mass spectrometric imaging with colloidal silver. Rapid Communications in Mass Spectrometry. 24:1147-1154. abstract
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