Cleaning Vials

  • Put on gloves and aquire two 600 mL containers (one labeled soap, one labeled water) and the vials/flasks to be cleaned.
  • Use pure water from the Milli-Q purification machine.
  • Rinse containers and put the vials in the soap container.
  • Pour a small (2% of total volume) amount of Hellmanex cleaner into the soap container and fill.
  • Use necessary precautions associated with Hellmanex.
  • Put the soap container in the sonicator and fill the bath.
  • Turn it on and clean the vials for 10 minutes.
  • Empty out the container and rinse the vials a few times.
  • Refill the soapy container with water, no Hellmanex and sonicate for anther 10 minutes.
  • Empty the container again, rinse the vials, and place them into the water container, fill.
  • Repeat sonication and rinsing a few more times.
  • Dry the vials in the oven at ~100C.
  • When they are dry, rinse once with methanol and dry again.
  • Your vials are now ready to use.