Cleaning Trough and Barrier

Cleaning the Trough

  • Put on gloves and fill a 1 liter bottle with pure water from the Milli-Q purifier.
  • Remove cover of the trough and the barrier. To clean the barrier, please see below.
  • Pour water into the trough until it is just full, i.e. not bulging over.
  • Add Hellmanex soap, 2 milliliters is a good amount.
  • If the trough is temperature regulated, turn the thermostat up to about 40 degrees Celsius.
  • Let the water sit for at least 30 minutes.
  • Empty and rinse the trough 6 times to make sure the Hellmanex is gone and the trough is clean.

Cleaning the Barrier

  • The barrier should first be cleaned with ethanol and rinsed with pure water.
  • Place the barrier in a rather large, clean, plastic container filled with pure water from the Milli-Q purifier.
  • Add Hellmanex soap, about 2 milliliters, to the water.
  • Place this container in the sonicator, which should be filled with pure water also. Set it for 15 minutes.
  • After it is done, rinse out the container and barrier with pure water.
  • Fill the container again with pure water and insert the barrier again.
  • Put the container with the barrier in it in the sonicator again for another 15 minutes.
  • Once it is done, rinse out the container and barrier again.
  • The barrier is now clean, you are ready to insert it into a clean trough.