Wavelength-Dependent Differential Interference Contrast Microscopy: Multiplexing Detection Using Nonfluorescent Nanoparticles

TitleWavelength-Dependent Differential Interference Contrast Microscopy: Multiplexing Detection Using Nonfluorescent Nanoparticles
Publication TypeJournal Article
Year of Publication2010
AuthorsLuo Y, Sun W, Gu Y, Wang GF, Fang N
Journal TitleAnalytical Chemistry
Volume82
Pages6675-6679
Date Published8/1
ISBN Number0003-2700
Accession NumberISI:000280401400051
KeywordsDNA, raman-scattering, spectroscopy
Abstract

The wavelength dependence of plasmonic nanoparticles' contrasts in differential interference contrast (DIC) microscopy has been exploited previously for unambiguous identification and dynamic tracking of these nanoprobes in complex environments (Anal. Chem. 2009, 81, 9203-9208). In the present study, the suitability of multiplexing detection in DIC microscopy was investigated systematically with 19 kinds of nanoparticles of different materials and/or sizes. A unique DIC contrast spectrum was found for each kind of nanoparticle. Multiplexing detection was accomplished by measuring DIC contrasts at a minimum of two specific illumination wavelengths. The main advantages of DIC microscopy for multiplexing detection over other nonfluorescence techniques, such as dark field microscopy and surface-enhanced Raman scattering, were demonstrated by differentiating four kinds of nanopartides on the cell membrane while providing high-contrast images of both the nanoprobes and cell features.

URL<Go to ISI>://000280401400051
DOIDoi 10.1021/Ac101336d