Single DNA molecules as probes for interrogating silica surfaces after various chemical treatments

TitleSingle DNA molecules as probes for interrogating silica surfaces after various chemical treatments
Publication TypeJournal Article
Year of Publication2007
AuthorsLiu X, Wu Z, Nie H, Liu Z, He Y, Yeung ES
Journal TitleAnalytica Chimica Acta
Volume602
Pages229-235
Date PublishedOct
Type of ArticleArticle
ISBN Number0003-2670
Accession NumberISI:000250629800010
Keywordsadsorption, adsorption glass surfaces, capillary-electrophoresis, diffusion, fluorescence, glass, interface, lambda-DNA, microscopy, PROTEIN MOLECULES, single-molecule imaging, total internal reflection, TOTAL INTERNAL-REFLECTION
Abstract

We examined the adsorption of single YOYO-1-labeled lambda-DNA molecules at glass surfaces after treatment with various chemical cleaning methods by using total internal reflection fluorescence microscopy (TIRFM). The characteristics of these surfaces were further assessed using contact angle (CA) measurements and atomic force microscopy (AFM). By recording the real-time dynamic motion of DNA molecules at the liquid/solid interface, subtle differences in adsorption affinities were revealed. The results indicate that the driving force for adsorption of DNA molecules on glass surfaces is mainly hydrophobic interaction. We also found that surface topography plays a role in the adsorption dynamics. (C) 2007 Elsevier B.V. All rights reserved.

DOI10.1016/j.aca.2007.09.020
Alternate JournalAnal. Chim. Acta