Selection of Haploid Maize Kernels from Hybrid Kernels for Plant Breeding Using Near-Infrared Spectroscopy and SIMCA Analysis

TitleSelection of Haploid Maize Kernels from Hybrid Kernels for Plant Breeding Using Near-Infrared Spectroscopy and SIMCA Analysis
Publication TypeJournal Article
Year of Publication2012
AuthorsJones RW, Reinot T, Frei UK, Tseng Y, Lubberstedt T, McClelland JF
Journal TitleApplied Spectroscopy
Volume66
Pages447-450
Date Published04
Type of ArticleArticle
ISBN Number0003-7028
Accession NumberWOS:000301760400006
KeywordsCorn, Haploid, independent modeling of class analogy, maize, Near-infrared spectroscopy, NIR spectroscopy, oil, Partial least squares, PLS, reflectance spectroscopy, selection, SIMCA, single corn kernels, Single kernel analysis, Soft, spectra, transmittance
Abstract

Samples of haploid and hybrid seed from three different maize donor genotypes after maternal haploid induction were used to test the capability of automated near-infrared transmission spectroscopy to individually differentiate haploid from hybrid seeds. Using a two-step chemometric analysis in which the seeds were first classified according to genotype and then the haploid or hybrid status was determined proved to be the most successful approach. This approach allowed 11 of 13 haploid and 25 of 25 hybrid kernels to he correctly identified from a mixture that included seeds of all the genotypes.

URL<Go to ISI>://WOS:000301760400006
DOI10.1366/11-06426
Alternate JournalAppl. Spectrosc.