Recent advances in single-molecule detection on micro- and nano-fluidic devices

TitleRecent advances in single-molecule detection on micro- and nano-fluidic devices
Publication TypeJournal Article
Year of Publication2011
AuthorsLiu C, Qu YY, Luo Y, Fang N
Journal TitleElectrophoresis
Volume32
Pages3308-3318
Date Published11
Type of ArticleReview
ISBN Number0173-0835
Accession NumberWOS:000298300900002
Keywordscapillary-electrophoresis, channels, correlation spectroscopy, DETECTION, DNA-MOLECULES, Lab-on-a-chip, latest developments, liquid-solid interface, methacrylate microfluidic chip, Microfluidics, Miniaturization, nanofluidic, optical reconstruction microscopy, PROTEIN MOLECULES, SINGLE-MOLECULE, Ultra-sensitive detection, wave-guides
Abstract

Single-molecule detection (SMD) allows static and dynamic heterogeneities from seemingly equal molecules to be revealed in the studies of molecular structures and intra- and inter-molecular interactions. Micro- and nanometer-sized structures, including channels, chambers, droplets, etc., in microfluidic and nanofluidic devices allow diffusion-controlled reactions to be accelerated and provide high signal-to-noise ratio for optical signals. These two active research frontiers have been combined to provide unprecedented capabilities for chemical and biological studies. This review summarizes the advances of SMD performed on microfluidic and nanofluidic devices published in the past five years. The latest developments on optical SMD methods, microfluidic SMD platforms, and on-chip SMD applications are discussed herein and future development directions are also envisioned.

URL<Go to ISI>://WOS:000298300900002
DOI10.1002/elps.201100159
Alternate JournalElectrophoresis