Luciferase and Luciferin Co-immobilized Mesoporous Silica Nanoparticle Materials for Intracellular Biocatalysis

TitleLuciferase and Luciferin Co-immobilized Mesoporous Silica Nanoparticle Materials for Intracellular Biocatalysis
Publication TypeJournal Article
Year of Publication2011
AuthorsSun XX, Zhao YN, Lin VSY, Slowing II, Trewyn BG
Journal TitleJournal of the American Chemical Society
Volume133
Pages18554-18557
Date Published11/23
ISBN Number0002-7863
Accession NumberISI:000297398900014
Keywordsadenosine-triphosphate, cancer, delivery-system, firefly luciferase, glutathione, Gold nanoparticles, IN-VIVO, kinetics, proteins, responsive controlled-release
Abstract

We report a gold nanoparticle (AuNP)-capped mesoporous silica nanoparticle (Au-MSN) platform for intracellular codelivery of an enzyme and a substrate with retention of bioactivity. As a proof-of-concept demonstration, Au-MSNs are shown to release luciferin from the interior pores of MSN upon AuNP uncapping in response to disulfide-reducing antioxidants and codeliver bioactive luciferase from the PEGylated exterior surface of Au-MSN to Hela cells. The effectiveness of luciferase-catalyzed luciferin oxidation and luminescence emission in the presence of intracellular ATP was measured by a luminometer. Overall, the chemical tailorability of the Au-MSN platform to retain enzyme bioactivity, the ability to codeliver enzyme and substrate, and the potential for imaging tumor growth and metastasis afforded by intracellular ATP- and glutathione-dependent bioluminescence make this platform appealing for intracellular controlled catalysis and tumor imaging.

URL<Go to ISI>://000297398900014http://pubs.acs.org/doi/pdfplus/10.1021/ja2080168
DOIDoi 10.1021/Ja2080168
Alternate JournalJ Am Chem Soc