Imaging MS Methodology for More Chemical Information in Less Data Acquisition Time Utilizing a Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer

TitleImaging MS Methodology for More Chemical Information in Less Data Acquisition Time Utilizing a Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer
Publication TypeJournal Article
Year of Publication2010
AuthorsPerdian DC, Lee YJ
Journal TitleAnalytical Chemistry
Volume82
Pages9393-9400
Date Published11/15
ISBN Number0003-2700
Accession NumberISI:000284080500038
KeywordsARABIDOPSIS-THALIANA, atmospheric-pressure, brain-tissue, ELECTROSPRAY-IONIZATION, MATRIX, metabolites, proteins, SMALL MOLECULES, STRUCTURAL-CHARACTERIZATION, TISSUE-SECTIONS
Abstract

A novel mass spectrometric imaging method is developed to reduce the data acquisition time and provide rich chemical information using a hybrid linear ion trap-orbitrap mass spectrometer. In this method, the linear ion trap and orbitrap are used in tandem to reduce the acquisition time by incorporating multiple linear ion trap scans during an orbitrap scan utilizing a spiral raster step plate movement The data acquisition time was decreased by 43-49% in the current experiment compared to that of orbitrap-only scans; however, 75% or more time could be saved for higher mass resolution and with a higher repetition rate laser. Using this approach, a high spatial resolution of 10 mu m was maintained at ion trap imaging, while orbitrap spectra were acquired at a lower spatial resolution, 20-40 mu m, all with far less data acquisition time. Furthermore, various MS imaging methods were developed by interspersing MS/MS and MSn ion trap scans during orbitrap scans to provide more analytical information on the sample. This method was applied to differentiate and localize structural isomers of several flavonol glycosides from an Arabidopsis flower petal in which MS/MS, MSn, ion trap, and orbitrap images were all acquired in a single data acquisition.

URL<Go to ISI>://000284080500038http://pubs.acs.org/doi/pdfplus/10.1021/ac102017q
DOI10.1021/Ac102017q
Alternate JournalAnal Chem