Adsorption of single DNA molecules at the water/fused-silica interface

TitleAdsorption of single DNA molecules at the water/fused-silica interface
Publication TypeJournal Article
Year of Publication2007
AuthorsIsailovic S, Li HW, Yeung ES
Journal TitleJournal of Chromatography A
Volume1150
Pages259-266
Date PublishedMay
Type of ArticleProceedings Paper
ISBN Number0021-9673
Accession NumberISI:000247049200035
Keywordsadsorption, CAPILLARY ZONE ELECTROPHORESIS, DNA, dynamics, fused-silica, SINGLE-MOLECULE
Abstract

We applied total internal reflection fluorescence microscopy (TIRFM) to study intermolecular interactions at the water/fused-silica interface at the sm-le-molecule level. Real-time molecular motion at the interface was recorded to reveal adsorption behavior and conformational dynamics of three DNAs with sticky ends of different numbers of impaired bases. Features of DNA motion at the interface, such as evanescent-field residence time, linear velocity and frequency of adsorption/desorption events were measured to assess the relative affinities of the oligonucleotides for the surface. The general trend of stronger interaction with the surface for longer sticky ends confirmed hydrophobic interaction and hydrogen bonding as the driving forces of DNA adsorption to fused-silica at pH5. For DNAs of different sizes, different conformational dynamics and the accessibility of sticky ends give rise to a nonlinear relationship with respect to affinity. Such information may prove valuable for chromatography studies as well as for the design of DNA microarrays and drug delivery systems. (C) 2006 Elsevier B.V. All rights reserved.

DOI10.1016/j.chroma.2006.09.093
Alternate JournalJ. Chromatogr. A