Chemical Analysis of Nanodomains


Project Leader(s):
Emily Smith

Principal Investigators:
Ning Fang, Jacob Petrich, Emily Smith


We seek to understand the basic principles that underlie energy-relevant chemical separations; develop analytical methods to improve the sensitivity, reliability, and productivity of analytical determinations; and to develop new approaches to analysis. Our research emphasizes instrumentation and technique development highly relevant to the main focus areas of the Separation and Analysis activities of the Division of Chemical Science, Geoscience and Biosciences within the DOE Office of Basic Energy Sciences.  

The goal of this research is to develop the next generation of imaging tools and methodologies for the analysis of phenomena that occur at nanometer length scales and picosecond time scales. The developed instrumentation and methodology will be applied to model systems of interest to the DOE mission, where fundamental insight can be gained with the high spatial and temporal resolution afforded by our developed methods: chemical reac tions in heterogeneous silica supported catalysts; the organization and dynamics of mixed model lipid bilayers and cell membranes; chromatographic interactions; and heterogeneous enzyme reactions. The methods we propose to develop are:

1. High resolution total internal reflection (TIR) Raman microspectroscopy and imaging
2. Sub-diffraction limited imaging, including differential interference contrast (DIC) microscopy, variable-angle evanescent-field (EFM) microscopy, and time-resolved stimulated emission depletion (STED) microscopy
3. Novel single molecule spectroscopies


This research is supported by the U.S. Department of Energy, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences through the Ames Laboratory.  The Ames Laboratory is operated for the U.S. Department of Energy by Iowa State University under Contract No. DE-AC02-07CH11358.


Luo Y; Sun W; Gu Y; Wang G F; Fang N . 2010. Wavelength-Dependent Differential Interference Contrast Microscopy: Multiplexing Detection Using Nonfluorescent Nanoparticles. Analytical Chemistry. 82:6675-6679. abstract
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Stender A S; Wang G F; Sun W; Fang N . 2010. Influence of Gold Nanorod Geometry on Optical Response. ACS Nano. 4:7667-7675. abstract
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Wang G F; Sun W; Luo Y; Fang N . 2010. Resolving Rotational Motions of Nano-objects in Engineered Environments and Live Cells with Gold Nanorods and Differential Interference Contrast Microscopy. Journal of the American Chemical Society. 132:16417-16422. abstract
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Ma C B; Yeung E S . 2010. Highly sensitive detection of DNA phosphorylation by counting single nanoparticles. Analytical and Bioanalytical Chemistry. 397:2279-2284. abstract
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Mckee K J; Smith E A . 2010. Development of a scanning angle total internal reflection Raman spectrometer. Review of Scientific Instruments. 81:043106. abstract
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Sun W; Marchuk K; Wang G F; Fang N . 2010. Autocalibrated Scanning-Angle Prism-Type Total Internal Reflection Fluorescence Microscopy for Nanometer-Precision Axial Position Determination. Analytical Chemistry. 82:2441-2447. abstract
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Liu C; Luo Y; Maxwell E J; Fang N; Chen D D Y . 2010. Reverse of Mixing Process with a Two-Dimensional Electro-Fluid-Dynamic Device. Analytical Chemistry. 82:2182-2185. abstract
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Ma C B; Yeung E S . 2010. Entrapment of Individual DNA Molecules and Nanoparticles in Porous Alumina Membranes. Analytical Chemistry. 82:654-657. abstract
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Ma C B; Yeung E S . 2010. Single Molecule Imaging of Protein Molecules in Nanopores. Analytical Chemistry. 82:478-482. abstract
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Kang S H; Lee S; Yeung E S . 2010. Detection of Single Enzyme Molecules inside Nanopores on the Basis of Chemiluminescence. Angewandte Chemie-International Edition. 49:2603-2606. abstract
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