Chemical Analysis of Nanodomains


Project Leader(s):
Emily Smith

Principal Investigators:
Ning Fang, Jacob Petrich, Emily Smith


We seek to understand the basic principles that underlie energy-relevant chemical separations; develop analytical methods to improve the sensitivity, reliability, and productivity of analytical determinations; and to develop new approaches to analysis. Our research emphasizes instrumentation and technique development highly relevant to the main focus areas of the Separation and Analysis activities of the Division of Chemical Science, Geoscience and Biosciences within the DOE Office of Basic Energy Sciences.  

The goal of this research is to develop the next generation of imaging tools and methodologies for the analysis of phenomena that occur at nanometer length scales and picosecond time scales. The developed instrumentation and methodology will be applied to model systems of interest to the DOE mission, where fundamental insight can be gained with the high spatial and temporal resolution afforded by our developed methods: chemical reac tions in heterogeneous silica supported catalysts; the organization and dynamics of mixed model lipid bilayers and cell membranes; chromatographic interactions; and heterogeneous enzyme reactions. The methods we propose to develop are:

1. High resolution total internal reflection (TIR) Raman microspectroscopy and imaging
2. Sub-diffraction limited imaging, including differential interference contrast (DIC) microscopy, variable-angle evanescent-field (EFM) microscopy, and time-resolved stimulated emission depletion (STED) microscopy
3. Novel single molecule spectroscopies


This research is supported by the U.S. Department of Energy, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences through the Ames Laboratory.  The Ames Laboratory is operated for the U.S. Department of Energy by Iowa State University under Contract No. DE-AC02-07CH11358.


Liu C; Qu Y Y; Luo Y; Fang N . 2011. Recent advances in single-molecule detection on micro- and nano-fluidic devices. Electrophoresis. 32:3308-3318. abstract
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Meyer M W; Smith E A . 2011. Optimization of silver nanoparticles for surface enhanced Raman spectroscopy of structurally diverse analytes using visible and near-infrared excitation. Analyst. 136:3542-3549. abstract
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Lee S; Kang S H; Yeung E S . 2011. Enzyme digestion of entrapped single-DNA molecules in nanopores. Talanta. 85:2135-2141. abstract
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Sun W; Xu A S; Marchuk K; Wang G F; Fang N . 2011. Whole-Cell Scan Using Automatic Variable-Angle and Variable-Illumination-Depth Pseudo-Total Internal Reflection Fluorescence Microscopy. JALA. 16:255-262. abstract
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Luo Y; Sun W; Liu C; Wang G F; Fang N . 2011. Superlocalization of Single Molecules and Nanoparticles in High-Fidelity Optical Imaging Microfluidic Devices. Analytical Chemistry. 83:5073-5077. abstract
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Ha J W; Sun W; Wang G F; Fang N . 2011. Differential interference contrast polarization anisotropy for tracking rotational dynamics of gold nanorods. Chemical Communications. 47:7743-7745. abstract
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Gu Y; Sun W; Wang G F; Fang N . 2011. Single Particle Orientation and Rotation Tracking Discloses Distinctive Rotational Dynamics of Drug Delivery Vectors on Live Cell Membranes. Journal of the American Chemical Society. 133:5720-5723. abstract
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Shih C J; Lupoi J S; Smith E A . 2011. Raman spectroscopy measurements of glucose and xylose in hydrolysate: Role of corn stover pretreatment and enzyme composition. Bioresource Technology. 102:5169-5176. abstract
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Liu C; Luo Y; Fang N; Chen D D Y . 2011. Analyte Distribution at Channel Intersections of Electro-Fluid-Dynamic Devices. Analytical Chemistry. 83:1189-1192. abstract
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Yeung E S . 2011. Genome-wide Correlation between mRNA and Protein in a Single Cell. Angewandte Chemie-International Edition. 50:583-585. abstract
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