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Integrated and Dynamic Multi-Spectroscopic in situ Imaging of Plant Metabolism at the Level of Subcellular Compartments

Additional Principal Investigators: Diane Bassham, Arthur Winter, Eve Wurtele, Basil Nikolau (Iowa State University)

This research team is developing novel, integrated, multi-spectral molecular imaging technologies that will assess and dynamically image metabolic processes that are asymmetrically distributed at the cellular and subcellular levels of plant organs. The molecular imaging technologies include Raman and subdiffraction fluorescence imaging, imaging with dynamic fluorescent, and Raman molecular probes and mass spectrometric imaging. 

The Ames Laboratory work is focused on optical imaging. The novel molecular imaging capabilities will be computationally unified with traditional high-resolution visual imaging techniques by adapting the existing, award-winning dynamic visualization Meta!Blast platform.

This research is supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research (BER) through the Ames Laboratory. The Ames Laboratory is operated for the U.S. Department of Energy by Iowa State University under Contract No. DE-AC02-07CH11358

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