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Imaging Gene Expression

FWP/Project Description:

Project Leader(s): Marit Nilsen-Hamilton

Principal Investigators: George Kraus

 

We are developing apatmers to use as cellular receptors for imaging gene expression in a technology termed Gene expression measurement by Revealed Aptamer Based Imaging Technology (GRABIT) that includes two approaches using aptamers to image gene expression:  (i) Intracellular Multiaptamer Genetic tags (IMAGEtags), which are aptamer expressing reporter genes, and (ii) Targeted Reversibly Attenuated Probes (TRAPS), which are allosterically regulated aptamer probes.  These RNA probes are being developed for imaging in plants and bacterial species.  

This research is supported by the U.S. Department of Energy, Office of Biological and Environmental Research through the Ames Laboratory.  The Ames Laboratory is operated for the U.S. Department of Energy by Iowa State University under Contract No. DE-AC02-07CH11358