Imaging Gene Expression


Project Leader(s):
Marit Nilsen-Hamilton

Principal Investigators:
George Kraus


We are developing apatmers to use as cellular receptors for imaging gene expression in a technology termed Gene expression measurement by Revealed Aptmaer Based Imaging Technology (GRABIT) that includes tow approaches using aptamers to image gene expression:  (i) Intracellular Multiaptamer Genetic tags (IMAGEtags), which are aptamer expressing reporter genes, and (ii) Targeted Reversibly Attenuated Probes (TRAPS), which are allosterically regulated aptamer probes.  These RNA probes are being developed for imaging in plants and bacterial species.  


This research is supported by the U.S. Department of Energy, Office of Biological and Environmental Research through the Ames Laboratory.  The Ames Laboratory is operated for the U.S. Department of Energy by Iowa State University under Contract No. DE-AC02-07CH11358.


Ilgu M; Wang T J; Lamm M H; Nilsen-Hamilton M . 2013. Investigating the malleability of RNA aptamers. Methods. 63:178-187. abstract
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Nath Sangeeta; Spencer Virginia A; Han Ju; Chang Hang; Zhang Kai; Fontenay Gerald V; Anderson Charles; Hyman Joel M; Nilsen-Hamilton M; Chang Young-Tae; Parvin Bahram . 2012. Identification of Fluorescent Compounds with Non-Specific Binding Property via High Throughput Live Cell Microscopy. PLoS ONE. 7:e28802. abstract
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Wang T J; Hoy J A; Lamm M H; Nilsen-Hamilton M . 2009. Computational and Experimental Analyses Converge to Reveal a Coherent Yet Malleable Aptamer Structure That Controls Chemical Reactivity. Journal of the American Chemical Society. 131:14747-14755. abstract
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