2012 Iowa Regional Middle School Teams
| Ames |
| Anthon-Oto-Maple Valley |
| Dike-New Hartford |
| Eastern Iowa Home Schools (Cedar Rapids) |
| Evans (Ottumwa) |
| Harding (Cedar Rapids) |
| IKM Manning |
| LeMars |
| Madrid |
| Mediapolis |
| Notre Dame (Burlington) |
| Pella Christian |
| Saint Albert (Council Bluffs) |
| South Hamilton (Jewell) |
| Stilwell (West Des Moines) |
| Williamsburg |
